Luteolin Induces Cytotoxicity in Mix Cellularity Classical Hodgkin's Lymphoma via Caspase Activated-cell Death

Authors

Rajendra Gharbaran, CUNY Bronx Community CollegeFollow
Enyuan Shang, CUNY Bronx Community CollegeFollow
Onyekwere Onwumere, CUNY Lehman CollegeFollow
Naomi Codrington, CUNY Lehman CollegeFollow
Evangelina Dankwa Sarpong, CUNY Bronx Community College
Stephen M. Redenti, CUNY Lehman CollegeFollow

Document Type

Article

Publication Date

9-2020

Abstract

Background/aim: We investigated the effects of luteolin (LUT) on classical Hodgkin's lymphoma (cHL), since such studies in malignant lymphomas are lacking.

Materials and methods: Effect of LUT on cell growth was assessed with water-soluble tetrazolium 1 (WST-1) cell proliferation assay and automated hemocytometry on trypan blue-exclusion assay. Cell death was investigated with acridine orange/ethidium bromide live-dead assay, propidium iodide (PI) flow cytometry, and Annexin-V-PI microscopy. Caspase activation was studied using CellEvent Caspase-3/7 Green detection reagent. High resolution immunofluorescence microscopy was used to detect cleaved-PARP-1.

Results: LUT induced a dose-dependent decrease in the growth of KMH2 and L428 cells, cellular models of mix-cellularity (MC) and nodular sclerosis (NS) cHL, respectively. However, LUT induced cell death only in KMH2, at a higher concentration, and this was associated with caspase activation and cleaved PARP-1.

Conclusion: LUT induces cytotoxicity in the MC-cHL cellular model KMH2 via caspase activation.

Comments

This work was originally published in Anticancer Research, available at DOI: 10.21873/anticanres.14493