Dissertations and Theses

Date of Award


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First Advisor

Mark Emerson


Many human eye conditions result in the loss of vision, due to the degeneration of cone photoreceptors in the retina. Thus, the development of methods to regenerate these cone photoreceptors is an active area of study. In order to find ways to regenerate cones, the molecular pathways and gene regulatory networks that a retinal progenitor cell takes to develop into a postmitotic cone photoreceptor needs to be well understood. In an attempt to answer this question, this paper studies the effect of CRISPR/Cas9-mediated mutagenesis of two key transcription factors, Otx2 and Nr2e3, on retinal progenitor cells and cone photoreceptors throughout the development of the retina. This study also explores optimization of the CRISPR/Cas9 system to target specific cell populations.

Available for download on Wednesday, May 22, 2024

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