Date of Degree
Neepa T. Maitra
Luat T. Vuong
Atomic, Molecular and Optical Physics | Biological and Chemical Physics
Second Harmonic Generation, Microscopy, Retinal Imaging, Microtubules, Nonlinear Optics, Polarization Resolved Second Harmonic Generation
Here we present the utilization of Second Harmonic Generation (SHG) for label-free imaging of microtubules (MTs) in the retinal nerve fiber layer (RNFL). MTs are an important part of axonal cytoskeleton, providing structural support and serving as a railroad in intracellular transport. We demonstrate the application of SHG microscopy to the following studies: 1) Can changes in MT conformation be detected when treated with a stabilizing drug (Taxol); 2) if disruption in MT precedes loss of axons in a mouse model of glaucoma (DBA/2J); and 3) if elevated levels of intraocular pressure affect MT integrity. Our results validate SHG imaging as a promising tool to research the biophysics of MTs and its possible role in neurodegenerative disorders
Sharoukhov, Denis Y., "Second Harmonic Generation – A Novel Approach in Retinal Imaging" (2017). CUNY Academic Works.
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