Date of Award

Summer 9-2-2024

Document Type

Thesis

Degree Name

Master of Arts (MA)

Department

Chemistry

First Advisor

Yujia Xu

Second Advisor

Nathan Astrof

Third Advisor

Paul Feinstein

Academic Program Adviser

Yujia Xu

Abstract

A major challenge of developing collagen mimetic peptides (CMPs) by bacterial expression is to include hydroxyproline for biomedical applications. Coexpression of Prolyl-4-hydroxylase from A.mimivirus with CMPs was investigated. Additionally, four expression designs were created for potential applications in mammalian cells to utilize their natural ability of proline-hydroxylation by post-translational modification.

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