Date of Award
Fall 11-30-2019
Document Type
Thesis
Degree Name
Master of Science (MS)
Department/Program
Forensic Science
Language
English
First Advisor or Mentor
Richard Li
Second Reader
Artem Domashevskiy
Third Advisor
Elisa Wurmbach
Abstract
Currently, short tandem repeats are used in forensic DNA typing to identify the DNA profiles recovered from evidence samples and deceased individuals. Recently, SNPs that are related to pigmentation have been researched for their use in the identification of phenotypic traits. The development of a SNP panel that could successful identify the eye and skin color of an individual, would be immensely useful in missing person cases or mass disaster victim identification. Eight SNPs were selected into a SNaPshot assay that could accurately predict eye and skin color, five for eye color and six for skin color. Development of the SNaPshot assay begins with development of a multiplex PCR. A multiplex PCR of these eight SNPs was developed to be incorporated into this assay, so it can be used for identification of phenotypic traits. Primer pairs for each of the eight SNPs were tested in singleplex reactions for their accuracy and efficiency. Successful primers were subsequently pooled into multiplex reactions to determine which primers could function together in a multiplex reaction with accurate results.
Recommended Citation
Rodriguez, Stephanie A., "Development of Multiplex PCR Assay as a basis for Predicting Pigment Related Features" (2019). CUNY Academic Works.
https://academicworks.cuny.edu/jj_etds/132