Dissertations and Theses
Date of Award
2025
Document Type
Thesis
Department
Biology
First Advisor
Karen Hubbard
Second Advisor
Jonathan levitt
Third Advisor
Mark Emerson
Keywords
HnrnpA1, Hippocampus, Doxorubicin, cyclophosphamide, Chemotherapy induced cognitive impairment, Neurons
Abstract
This study investigated the molecular mechanisms underlying chemotherapy-induced cognitive impairment (CICI) following doxorubicin and cyclophosphamide treatment in a rat model, revealing three significant alterations in hippocampal tissue: selective downregulation of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) (21% decrease, p=0.0298), compromised astrocytic network integrity evidenced by reduced GFAP-positive area in cornu ammonis 3 (CA3) (24%, pB=0.026) and dentate gyrus (DG) (19%, pB=0.027) regions, and elevated cellular senescence marker p19Arf specifically in DG (69%, pB=0.033) and CA3 (27%, pB=0.024). The inverse relationship between hnRNPA1 reduction and p19Arf elevation suggests a potential mechanistic link between RNA dysregulation and senescence induction, with pronounced vulnerability in regions critical for adult neurogenesis and memory processing, thereby providing molecular targets for developing neuroprotective strategies to mitigate cognitive impairment in cancer patients undergoing chemotherapy.
Recommended Citation
Chen, Lijie, "Investigation of RNA-Binding Protein Expression Changes in the Hippocampus Following Doxorubicin and Cyclophosphamide Treatment" (2025). CUNY Academic Works.
https://academicworks.cuny.edu/cc_etds_theses/1194
Included in
Biology Commons, Cognitive Neuroscience Commons, Molecular and Cellular Neuroscience Commons
