Dissertations and Theses

Date of Award


Document Type




First Advisor

Anuradha Janakiraman

Second Advisor

Mark Emerson

Third Advisor

Shireen Saleque


Bacteria, Cytokinesis, FtsZ


In Escherichia coli, cell division is defined by the polymerization and constriction of a cytokinetic ring (Z ring) formed by FtsZ, a tubulin-like GTPase, at midcell. Division also involves the formation of a multi-protein complex at midcell known as the divisome. Several divisome proteins promote the assembly/disassembly processes of FtsZ, thereby exercising spatiotemporal control over division. Among FtsZ regulatory proteins are the FtsZ ringassociated proteins (Zap), which either directly or indirectly stabilize the Z-ring by increasing lateral interactions amongst FtsZ protofilaments in the Z-ring. ZapA-D are recruited during early cytokinesis and have overlapping functions in stabilizing FtsZ at midcell, but share no primary sequence identity. The focus of this study is on ZapC, a small cytoplasmic protein that is conserved in the gamma proteobacteria, co-localizes with FtsZ at midcell, and promotes lateral interactions amongst FtsZ protofilaments in the Z-ring. Cells lacking ZapC show modest increase in cell lengths and overexpression of ZapC leads to aberrant hyperstable Z-rings and lethal filamentation. In this study we attempt to address the precise molecular nature of how ZapC and FtsZ interact. Genetic, molecular and structural evidence from our work suggest that residues in a basic pocket-like domain of ZapC likely interact with conserved globular domain residues of FtsZ through electrostatic interactions. A better understanding of the FtsZ-ZapC interaction at atomic details could provide clues into the biology of Z-ring assembly and stability in E. coli and related species.


To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.