Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

Authors

Ryo Higuchi-Sanabria, Columbia University
Enrique J. Garcia, Columbia University
Delia Tomoiaga, CUNY Hunter CollegeFollow
Emilia L. Munteanu, Columbia University
Paul Feinstein, CUNY Hunter CollegeFollow
Liza A. Pon, Columbia University

Document Type

Article

Publication Date

1-4-2016

Abstract

Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/ mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging.

Comments

This article was originally published in PLoS one, available at doi:10.1371/journal. pone.0146120.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).