Date of Award

Spring 5-2020

Document Type

Thesis

Degree Name

Master of Science (MS)

Department/Program

Forensic Science

Language

English

First Advisor

Marta Concheiro-Guisan

Second Reader

Gail Cooper

Third Advisor

Garry Milman

Abstract

Due to recent changes in federal and state legislations, the availability and consumption of cannabis products have increased in the United States. The expanded use of recreational and medicinal cannabis products increases the importance of implementing sensitive and selective instrumental methods in toxicological laboratories, as legal implications may arise in forensic cases, such as driving under the influence of drugs (DUID). The purpose of this study was to perform a cross-validation for the quantitative analysis of cannabinoids (Δ9-tetrahydrocannabinol, cannabidiol, cannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol) in serum by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GCTQ). This method was fully validated following the Scientific Working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. Linearity was established within 1-100 ng/ml; bias was within ± 20% and imprecision was less than 20%; limits of detection (LOD) and quantitation (LOQ) were 1 ng/mL; and extraction efficiency ranged from 51.3 to 58.2%. Furthermore, the present method and a previously developed method for the determination of cannabinoids in blood were applied to serum (n = 20) and blood (n = 16) authentic case samples obtained from the New York City Office of the Chief Medical Examiner (NYC-OCME) to investigate a correlation between cannabinoid concentration in serum versus blood.

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