Breeding of the dog, Canis Iupus familiaris, over centuries has given rise to the selection of certain traits including size, shape, coat length, color and many behavioral traits. Inbreeding within dog breeds has resulted in the increase of genetic diseases, many of which are similar to human diseases making the dog an exemplary model organism to study. Using dCAPS (Derived Cleaved Amplified Polymorphic Sequences) we can identify SNPs (Single Nucleotide Polymorphisms) in the dog genome using buccal samples collected from four local dogs of different breeds, both large and small. After performing DNA extractions, PCR (Polymerase Chain Reaction), restriction enzyme digestions and gel electrophoresis on each sample, these genetic markers can be analyzed and differentiated.
Benmoshe, Nicole and Seto, Jeremy, "Genetic Diversity of Local Canines Assessed by Derived Cleaved Amplified Polymorphic Sequences" (2019). CUNY Academic Works.