Open-source computational framework for studying Drosophila behavioral phase

Authors

Boyan Kostadinov, CUNY New York City College of TechnologyFollow
Hannah Lee Pettibone, CUNY Graduate Center
Evardra Valerie Bell, Barnard College
Xiaona Zhou, CUNY New York City College of TechnologyFollow
Ausra Pranevicius, Barnard College
Orie Thomas Shafer, CUNY Graduate Center
Maria Paz Fernandez, Barnard CollegeFollow

Document Type

Article

Publication Date

3-19-2021

Abstract

This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an opensource computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided. For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020).

Comments

Kostadinov, B., Pettibone, H. L., Bell, E. V., Zhou, X., Pranevicius, A., Shafer, O. T., & Fernandez, M. P. (2021). Open-source computational framework for studying Drosophila behavioral phase. STAR protocols, 2(1), 100285, https://doi.org/10.1016/j.xpro.2020.100285

Published under a CC-BY-NC-ND 4.0 License.