Publications and Research

Document Type


Publication Date



This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an opensource computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided. For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020).


Kostadinov, B., Pettibone, H. L., Bell, E. V., Zhou, X., Pranevicius, A., Shafer, O. T., & Fernandez, M. P. (2021). Open-source computational framework for studying Drosophila behavioral phase. STAR protocols, 2(1), 100285,

Published under a CC-BY-NC-ND 4.0 License.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.