Accessibility is a key feature of usability. Providing web accessibility ensures that prospective users, particularly in science gateways are able to access advance resources for science research, educators, and students. The resources should be easily accessible in any site regardless of having a disability or not. Creating and implementing accessibility practices improves usability of sites for all users. The purpose of this study is to evaluate the Science Gateways for Accessible design. Previous research has been examined from selected science gateway sites (SGCI). In which results showed that none of these sites were fully compliant with W3C, WCAG 2.0 standards. We obtained quantitative data by creating a questionnaire and sent it to gateway organizations through an SGCI newsletter in order to examine their level of knowledge and interest in web accessibility. Our findings indicate that many of these organizations do have a strong interest in obtaining more knowledge on accessibility requirements and should dedicate more time to test for usability.