Date of Award
Summer 9-2-2024
Document Type
Thesis
Degree Name
Master of Arts (MA)
Department
Chemistry
First Advisor
Yujia Xu
Second Advisor
Nathan Astrof
Third Advisor
Paul Feinstein
Academic Program Adviser
Yujia Xu
Abstract
A major challenge of developing collagen mimetic peptides (CMPs) by bacterial expression is to include hydroxyproline for biomedical applications. Coexpression of Prolyl-4-hydroxylase from A.mimivirus with CMPs was investigated. Additionally, four expression designs were created for potential applications in mammalian cells to utilize their natural ability of proline-hydroxylation by post-translational modification.
Recommended Citation
Soldatich, Jennifer, "Developing coexpression systems to introduce hydroxyproline into protein engineered collagen peptides utilizing hydroxylase from Acanthamoeba polyphaga mimivirus" (2024). CUNY Academic Works.
https://academicworks.cuny.edu/hc_sas_etds/1232
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