Eukaryotic Initiation Factor 4E (eIF4E) in Complex With eIF4E Binding Protein 1 (4E-BP1) Binds With Higher Affinity to m7GpppN Cap of a Subset of Human mRNAs

Author

Izza F. Nawaz, CUNY Hunter CollegeFollow

Date of Award

Spring 4-26-2022

Document Type

Thesis

Degree Name

Master of Arts (MA)

Department

Chemistry

First Advisor

Dixie Goss

Second Advisor

Akira Kawamura

Academic Program Adviser

Yujia Xu

Abstract

Fluorescence anisotropy binding assays were used to analyze the binding of eIF4E in complex with 4E-BP1 onto the 5’ m7G cap of a subset of mRNA that are known to carry cap-independent translation. These studies suggest that 4E-BP1 increases eIF4E binding affinity to 5’cap of both FGF-9 and HIF-1𝝰.

Recommended Citation

Nawaz, Izza F., "Eukaryotic Initiation Factor 4E (eIF4E) in Complex With eIF4E Binding Protein 1 (4E-BP1) Binds With Higher Affinity to m7GpppN Cap of a Subset of Human mRNAs" (2022). CUNY Academic Works.
https://academicworks.cuny.edu/hc_sas_etds/851