Dissertations and Theses
Date of Award
2023
Document Type
Thesis
Department
Biology
First Advisor
Hysell V. Oviedo
Second Advisor
Mark Emerson
Third Advisor
Pinar Ayata
Keywords
mismatch repair, parvalbumin, connexin36, neural circuitry, primary auditory cortex
Abstract
DNA repair mechanisms are crucial for both cellular development and function. One highly conserved DNA repair factor is Mut-S Homolog 2 (Msh2), which corrects base-base mismatches and insertion/deletion loops. In humans, defects in this repair pathway are linked to diseases that have severe neurological pathologies. These include Lynch syndrome, Huntington’s disease and demyelination of the corpus callosum. The fundamental role of Msh2 in brain function is unknown. Using an Msh2-/- mouse model we began an exploration of its impact on the processing of sensory information, a crucial function to an animal’s survival. The goal of this study was to investigate potential deficits in thalamo-cortical function. Using sound stimulation and immunohistochemical detection of active neurons with the immediate early gene cfos, we observed a significant decrease in cortical and thalamic cfos expression in Msh2-/- mice, indicating a reduction in neural activity compared to WT littermates. To examine possible mechanisms underlying this hypoactivity, we focused on the thalamic reticular nucleus (TRN): a critical regulator of corticothalamic and thalamocortical activity. We observed an increase in parvalbumin (PV+) expression, density, and cell area in the TRN, as well as high aggregation of the gap junction protein connexin-36 (Cx36). Cx36 is required for electrical coupling between TRN neurons, regulating the degree of synchrony in firing patterns. This indicates that Cx36- expressing PV+ cells in the TRN undergo increased gap junction coupling, thus leading to dysfunction in the electrical properties of the TRN, and thalamo-cortical sensory transmission. These findings suggest that Msh2, and therefore mismatch-repair, plays a role in the regulation or development of inhibitory interneurons in the TRN, leading to a downstream decrease of sound evoked neural activity.
Recommended Citation
Rahman, Sadia N., "Auditory Processing Deficits in MSH2-KO mice are linked to Aberrant Inhibitory Neuron Function in the Thalamic Reticular Nucleus" (2023). CUNY Academic Works.
https://academicworks.cuny.edu/cc_etds_theses/1113