An Optical Aptamer-Based Carbon Nanosensor for Cortisol Detection

Author

Jessica Kretli Zanetti, CUNY City CollegeFollow

Date of Award

2024

Document Type

Thesis

Department

Biomedical Engineering

First Advisor

Ryan M. Williams

Keywords

Cortisol, Single-Walled Carbon Nanotubes (SWCNTs), Optical Sensor, Hormone Measurement, NIR (Near-Infrared) Spectroscopy

Abstract

Cortisol is a hormone related to numerous biological functions. This hormone is primarily associated with regulating the body’s stress response, including its effects on metabolism, immune function, and energy balance. Additionally, it plays a critical role in maintaining physiological homeostasis during periods of physical or psychological stress. Consequently, it is critical to monitor levels of this hormone to understand different vital processes it can impact. This work presents the development of an optical aptamer-based carbon nanosensor for the detection of cortisol. The sensor utilizes single-walled carbon nanotubes (SWCNTs) functionalized with a specific DNA aptamer to achieve high specificity and sensitivity. This work touches on the importance of solvent selection in preserving the optical properties of SWCNTs while allowing for effective hormone solubilization. Results indicate that the sensor can detect cortisol concentrations from 37.5 μg/mL to 300 μg/mL and can distinguish cortisol from estrogen. Challenges remain in detecting lower cortisol concentrations relevant for clinical applications, necessitating further optimization of the sensor's sensitivity. This research can lead to more rapid and lower cost point-of-care testing for cortisol, ultimately improving health outcomes.

Recommended Citation

Kretli Zanetti, Jessica, "An Optical Aptamer-Based Carbon Nanosensor for Cortisol Detection" (2024). CUNY Academic Works.
https://academicworks.cuny.edu/cc_etds_theses/1277