Dissertations and Theses

Date of Award

2024

Document Type

Thesis

Department

Chemical Engineering

First Advisor

Lane Gilchrist

Keywords

Breast cancer, Gamma Secretase, Lipids, Cell Culture

Abstract

Triple Negative Breast Cancer (TNBC) continues to be a formidable challenge in oncology, characterized by its aggressive phenotype and limited treatment options. Alarming racial disparities in TNBC outcomes persist, with underrepresented populations experiencing disproportionately higher rates of mortality and poorer prognoses. This master's thesis aims to delve into the molecular intricacies that contribute to these disparities, with a specific focus on gamma secretase activity, a key regulator of cellular processes.

The research methodology encompasses a multi-faceted approach, amalgamating clinical data analysis with extensive in vitro and in vivo experimentation. The study employs cutting-edge molecular profiling techniques and gene expression analysis to discern potential variations in gamma secretase activity across diverse racial cohorts afflicted with TNBC. This comprehensive analysis seeks to identify specific molecular signatures that may underlie the observed racial disparities in TNBC outcomes.

Furthermore, the project investigates the therapeutic potential of targeting gamma secretase to ameliorate racial disparities in TNBC. Utilizing advanced experimental models, including patient-derived cell lines representative of various racial backgrounds, the study evaluates the impact of gamma secretase inhibitors on TNBC progression. The goal is to elucidate potential differences in treatment response and explore the feasibility of gamma secretase inhibition as a targeted therapeutic strategy.

The anticipated outcomes of this research extend beyond the mere identification of molecular disparities, aiming to contribute valuable insights that can inform the development of tailored therapeutic interventions. By unravelling the complex interplay of molecular factors contributing to racial disparities in TNBC, this thesis seeks to pave the way for more equitable and effective treatment strategies, ultimately improving outcomes for individuals facing this aggressive form of breast cancer.

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