Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

Authors

Lingyan Shi, CUNY City College
Luyao Lu, Wenzhou Medical University
George Harvey, Riverdale Country School
Thomas Harvey, Riverdale Country School
Adrián Rodríguez-Contreras, CUNY City CollegeFollow
Robert R. Alfano, CUNY City CollegeFollow

Document Type

Article

Publication Date

6-1-2017

Abstract

In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.

Comments

This article was originally published in Scientific Reports, available at DOI:10.1038/s41598-017-02673-5.

This article is licensed under a Creative Commons Attribution 4.0 International License.