Lighting the way: Recent insights into the structure and regulation of phototropin blue light receptors

Authors

Jaynee E. Hart, CUNY Advanced Science Research CenterFollow
Kevin H. Gardner, CUNY City CollegeFollow

Document Type

Article

Publication Date

3-26-2021

Abstract

The phototropins (phots) are light-activated kinases that are critical for plant physiology and the many diverse optogenetic tools that they have inspired. Phototropins combine two bluelight- sensing Light–Oxygen–Voltage (LOV) domains (LOV1 and LOV2) and a C-terminal serine/threonine kinase domain, using the LOV domains to control the catalytic activity of the kinase. While much is known about the structure and photochemistry of the light-perceiving LOV domains, particularly in how activation of the LOV2 domain triggers the unfolding of alpha helices that communicate the light signal to the kinase domain, many questions about phot structure and mechanism remain. Recent studies have made progress addressing these questions by utilizing small-angle X-ray scattering (SAXS) and other biophysical approaches to study multidomain phots from Chlamydomonas and Arabidopsis, leading to models where the domains have an extended linear arrangement, with the regulatory LOV2 domain contacting the kinase domain N-lobe. We discuss this and other advances that have improved structural and mechanistic understanding of phot regulation in this review, along with the challenges that will have to be overcome to obtain high-resolution structural information on these exciting photoreceptors. Such information will be essential to advancing fundamental understanding of plant physiology while enabling engineering efforts at both the whole plant and molecular levels.

Comments

This article was originally published in the Journal of Biological Chemistry, available at https://doi.org/10.1016/j.jbc.2021.100594.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).