Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent chlorophyll α in spinach leaf

Authors

Lingyan Shi, CUNY City College
Adrián Rodríguez-Contreras, CUNY City College
Yury Budansky, CUNY City College
Yang Pu, CUNY City College
Thien An Nguyen, CUNY City College
Robert R. Alfano, CUNY City College

Document Type

Article

Publication Date

June 2014

Abstract

Abstract. Two-photon (2P) excitation of the second singlet (S2) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the “tissue optical window” (650 to 950 nm). S2 state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S2 state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.

Comments

This work was originally published in Journal of Biomedical Optics, available at doi:10.1117/1.JBO.19.6.066009.