Dissertations, Theses, and Capstone Projects
Date of Degree
5-2019
Document Type
Dissertation
Degree Name
Ph.D.
Program
Biology
Advisor
Stephen Redenti
Committee Members
Hyungsik Lim
Daniel Casper
Dianne Cox
Konstantinos Krampis
Subject Categories
Bioinformatics | Disease Modeling | Endocrine System Diseases | Eye Diseases | Molecular and Cellular Neuroscience | Molecular Biology | Nervous System Diseases
Keywords
extracellular vesicles, diabetes, diabetic retinopathy, proteomics, next generation sequencing, biomarker
Abstract
Diabetic Retinopathy (DR) is a neurovascular complication associated with diabetes mellitus that affects approximately 120 million people worldwide and its prevalence is expected to reach 190 million by 2030. DR diagnosis is accomplished with fundus ophthalmoscopy often when retinal damage and vision loss have already occurred. A group of biomarker being explored for early detection of diseases are extracellular vesicles (EVs), which are nanometer diameter lipid enclosed vesicles, released from all cell types and containing genetic cargo reflective of releasing cell state. EV biomarkers are currently being explored to help monitor disease predisposition, pathogenesis and response to treatment. While an increasing number of studies are analyzing EVs in the brain, EV morphology, release rates and content have yet to be elucidated in retinal disease. The approach to characterizing retinal EVs in this work is based on the premise that unique aspects of DR retinal cell expression patterns can be detected in retinal EV release rate and genetic cargo. This initial work has identified molecular signatures that have been shown to be involved in DR pathogenesis to be present in EVs and may be built on in future studies to develop a biomarker for early detection of DR prior to retinal damage and vision loss.
Recommended Citation
Mighty, Jason, "Characterization of Extracellular Vesicles from Human Diabetic Retinopathy Retinal Tissue in Vitro and from Urine of Human Patients with Diabetic Retinopathy" (2019). CUNY Academic Works.
https://academicworks.cuny.edu/gc_etds/3235
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Included in
Bioinformatics Commons, Disease Modeling Commons, Endocrine System Diseases Commons, Eye Diseases Commons, Molecular and Cellular Neuroscience Commons, Molecular Biology Commons, Nervous System Diseases Commons