Dissertations, Theses, and Capstone Projects
Date of Degree
2-2023
Document Type
Dissertation
Degree Name
Ph.D.
Program
Chemistry
Advisor
Maria Contel
Committee Members
Rein Ulijn
Aneta Mieszawska
Stephen O'Brien
Subject Categories
Analytical Chemistry | Inorganic Chemistry
Keywords
Peptide self-assembly, metal-based compounds, cancer, encapsulation, N-Heterocyclic carbene, MMP-9
Abstract
Enzyme-responsive materials have been well explored, particularly as therapeutic and diagnostic agents. In this thesis we demonstrate that anionic self-assembling peptides can be utilized as delivery vehicles for metal-based hydrophobic payloads. The tunability of the system is highlighted as well as the increase in cytotoxicity and selectivity in vitro. The rapid degradation of peptides in cell media may lead to the formation of new peptide-drug bioconjugates with increased activity and selectivity. The physiological stability of these peptide delivery vehicles has been optimized by capping the N-terminus with an acetyl group. This simple backbone modification was shown to not prevent self-assembly, the ability to load hydrophobic payloads, or modify the anticancer activity in vitro. This modification decreases peptide recognition by non-specific proteases, while retaining specificity towards an enzyme of interest (MMP-9). This highlights its potential as a stable enzyme-responsive delivery system.
Recommended Citation
Marciano, Yaron, "Optimized Peptide Nanomaterials as Delivery Vehicles for Hydrophobic Metal-Based Anticancer Agents" (2023). CUNY Academic Works.
https://academicworks.cuny.edu/gc_etds/5151