The 3′ processing factor CstF functions in the DNA repair response

Authors

Nurit Mirkin, CUNY Hunter College
Danae Fonseca, CUNY Hunter College
Samia Mohammed, CUNY Hunter College
Murat A. Cevher, CUNY Hunter College
James L. Manley, CUNY Hunter College
Frida E. Kleiman, CUNY Hunter CollegeFollow

Document Type

Article

Publication Date

April 2008

Abstract

Following DNA damage, mRNA levels decrease, reflecting a coordinated interaction of the DNA repair, transcription and RNA processing machineries. In this study, we provide evidence that transcription and polyadenylation of mRNA precursors are both affected in vivo by UV treatment. We next show that the polyadenylation factor CstF, plays a direct role in the DNA damage response. Cells with reduced levels of CstF display decreased viability following UV treatment, reduced ability to ubiquitinate RNA polymerase II (RNAP II), and defects in repair of DNA damage. Furthermore, we show that CstF, RNAP II and BARD1 are all found at sites of repaired DNA. Our results indicate that CstF plays an active role in the response to DNA damage, providing a link between transcription-coupled RNA processing and DNA repair.

Comments

This work was originally published in Nucleic Acids Research, available at doi:10.1093/nar/gkn005.