A prospective observational study was conducted at three ophthalmic clinics to assess the clinical patterns and etiology of uveitis in the Central Asia Republic of Tajikistan. All patients underwent physical and ophthalmologic examinations. Extensive efforts were made to determine the etiology of uveitis, including workups for Behçet's Disease (BD) and Vogt–Koyanagi–Harada (VKH) syndrome. All patients were tested for the presence of HLA-B27. Presence and/or history of tuberculosis and syphilis were ruled out. HSV, CMV, Chlamydia trachomatis and HIV antibody titers were determined by ELISA. Uveitis was diagnosed in 51 patient ages 14 to 60 were with women accounted for 60.8%. Bilateral uveitis was presented in 74.5% cases and dominated in all age groups. Seven HLA-B27-positive patients had isolated uveitis without systemic manifestations and five had uveitis associated with spondyloarthritis (SpA). In total, uveitis was associated most commonly with SpA (23.5%), BD (9.8%), and VKH syndrome (5.9%). In 41.2% of uveitis cases, etiology was unknown. Conclusions: The overall clinical patterns and etiology of uveitis in Tajikistan are comparable to those reported from outside the Central Asia region. Bilateral uveitis dominated in all age groups with HLA-B27-positive uveitis present in 23.5% of patients and more common in men.