Date of Award

Spring 5-2025

Document Type

Thesis

Degree Name

Master of Science (MS)

Department/Program

Forensic Science

Language

English

First Advisor or Mentor

Angelique Corthals

Second Reader

Nicholas Petraco

Third Advisor

Syed Hoda

Abstract

Identifying sharp-force trauma in thermally altered human remains presents a significant challenge in forensic investigations, particularly in arson-related deaths where traditional autopsy techniques may be limited. This study explores the efficacy of histological methods in detecting sharp-force trauma on partially cremated remains using pig ribs (Sus scrofa) as a human analog. Simulated tool marks from a knife, screwdriver, and flathead screwdriver were inflicted at varying angles (60° and 90°) and subsequently exposed to high temperatures in an open fire setting. Histological analyses, including Hematoxylin and Eosin Y (H&E) and Gomori’s Prussian Blue staining, were employed to assess tissue structure and detect trace metal transfer from the tools. Results indicated that while thermal exposure caused substantial tissue degradation and disrupted the bone matrix, Prussian Blue staining successfully identified ferric iron deposits in some specimens, suggesting potential for toolmark detection post-cremation. Conversely, H&E staining showed structural disruption but limited trauma differentiation. The study underscores the challenges and partial effectiveness of histological approaches in forensic contexts involving thermal destruction and highlights the need for continued experimental research incorporating advanced imaging techniques to improve trauma detection in burnt or cremated remains.

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