Theses

Date of Award

2026

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

First Advisor

Stephen Redenti

Abstract

Post-mitotic neurons can reenter the cell cycle and begin cell division, but this process typically ends in apoptosis. In this work I will use systems pharmacology and machine learning to target cell cycle and survival genes in post-mitotic neurons to drive successful cell division. In the first part of this work, I have selected through published data genes involved in neuron cell cycle re-entry and apoptosis. I have then used the systems pharmacology database drug gene budger to identify drugs to direct the expression of these genes toward cell cycle and survival. I provide eight tables of key identified genes and drugs resulting from drug gene budger with predicted changes associated with cell cycle and survival. In the second part of this work, I have identified full gene expression sets of post-mitotic neuron rod cells and (mitotic) late retinal progenitor cells and have worked with a collaborator to use machine learning to perform combinatorial perturbations of gene sets to drive re-entry into cell cycle and survival. I have identified network genes predicted to facilitate post-mitotic neuron rod cells re-entry to cell cycle and transition into (mitotic) late retinal progenitor state. This work holds promise for application in neural regeneration.

Available for download on Wednesday, June 21, 2028

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