Document Type
Activity or Lab
Publication Date
Spring 5-15-2026
Abstract
This laboratory manual for SCB252: Fundamentals of Biotechniques provides an integrated, hands-on introduction to core concepts and methods in modern biological research using Drosophila melanogaster as a model organism. The manual emphasizes laboratory safety, research integrity, and responsible conduct of research, alongside foundational experimental skills. Students are guided through key biological techniques including fly husbandry, genetic crosses, mutant analysis, microscopy, DNA extraction, polymerase chain reaction (PCR), gel electrophoresis, sequencing technologies, and bioinformatics tools such as BLAST and genome databases. Advanced modules introduce gene structure annotation, RNA-seq data analysis, and primer design, while fluorescence microscopy and protein analysis techniques further expand technical proficiency.
The curriculum also integrates broader scientific themes, including virology, genetic engineering, cancer biology, and ethical considerations in biotechnology, fostering connections between laboratory practice and societal impact. Inquiry-based research projects and data analysis exercises support the development of experimental design, statistical reasoning, and scientific communication skills. Overall, this manual serves as a comprehensive resource for training students in fundamental biotechniques while preparing them for independent research and critical engagement with biological sciences.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.
CUNY OER Funding
CUNY OER Initiative
