Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophages

Authors

Urszula Golebiewska, CUNY Queensborough Community College
Jason G. Kay, Hospital for Sick Children
Thomas Masters, National University of Singapore
Sergio Grinstein, Hospital for Sick Children
Wonpil Im, University of Kansas
Richard W. Pastor, National Institutes of Health
Suzanne Scarlata, Stony Brook University
Stuart McLaughlin, Stony Brook University

Document Type

Article

Publication Date

September 2011

Abstract

Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching measurements on macrophages injected with fluorescent phosphatidylinositol 4,5-bisphosphate (PIP2) suggest that a barrier impedes the diffusion of plasma membrane PIP2 into and out of forming phagosomes.

Comments

This work was originally published in Molecular Biology of the Cell, available at doi:10.1091/mbc.E11-02-0114.