Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes

Authors

Xingyu Yin, Brookhaven National Laboratory
Alexander Scalia, Brookhaven National Laboratory
Ludmila Leroy, Brookhaven National Laboratory
Christina M. Cuttitta, CUNY College of Staten Island
Gina M. Polizzo, Brookhaven National Laboratory
Daniel L. Ericson, Brookhaven National Laboratory
Christian G. Roessler, Brookhaven National Laboratory
Olven Campos, Brookhaven National Laboratory
Millie Y. Ma, Brookhaven National Laboratory
Rakhi Agarwal, Brookhaven National Laboratory
Rick Jackimowicz, Brookhaven National Laboratory
Marc Allaire, Brookhaven National Laboratory
Allen M. Orville, Brookhaven National Laboratory
Robert M. Sweet, Brookhaven National Laboratory
Alexei S. Soares, Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973-5000, USA

Document Type

Article

Publication Date

May 2014

Abstract

A method is presented for screening fragment libraries using acoustic droplet ejection to co-crystallize proteins and chemicals directly on micromeshes with as little as 2.5 nl of each component. This method was used to identify previously unreported fragments that bind to lysozyme, thermolysin, and trypsin.

Comments

This work was originally published in Acta Crystallographica Section D: Biological Crystallography, available at doi:10.1107/S1399004713034603.